Dr Ezra Bruggeman

I am a biophysicist working on super-resolution fluorescence microscopy in the Lee Lab. My current work focuses on estimating the orientation of single molecules using optical microscopes.

Before I joined the Lee Lab in October 2019, I studied bioengineering at the University of Antwerp (Belgium) and biophysics at KU Leuven (Belgium) during which I worked on the development of a novel method for spectral single-molecule localization microscopy in the group of Prof. Peter Dedecker.

Publications

POLCAM: Instant molecular orientation microscopy for the life sciences

E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, AS Klymchenko, SJ Davis, EK Paluch, D Klenerman, MD Lew, K O’Holleran, SF Lee

(2023)

(doi: 10.1101/2023.02.07.527479)

Fast and artifact-free excitation multiplexing using synchronized image scanning

E Bruggeman, R Van den Eynde, B Amouroux, T Venneman, PV Berghe, M Müller, W Vandenberg, P Dedecker

(2023)

(doi: 10.1101/2023.02.07.527342)

resPAINT: Accelerating Volumetric Super‐Resolution Localisation Microscopy by Active Control of Probe Emission**

EW Sanders, AR Carr, E Bruggeman, M Körbel, SI Benaissa, RF Donat, AM Santos, J McColl, K O'Holleran, D Klenerman, SJ Davis, SF Lee, A Ponjavic

Angew Chem Weinheim Bergstr Ger

(2022)

134

e202206919

(doi: 10.1002/ange.202206919)

Quantification of the NA dependent change of shape in the image formation of a z‐polarized fluorescent molecule using vectorial diffraction simulations

F Ströhl, E Bruggeman, CJ Rowlands, DL Wolfson, BS Ahluwalia

Microscopy Research and Technique

(2022)

2016

(doi: 10.1002/jemt.24060)

EAP45 association with budding HIV-1: Kinetics and domain requirements

B Meng, PP Vallejo Ramirez, KM Scherer, E Bruggeman, JC Kenyon, CF Kaminski, AM Lever

Traffic

(2021)

439

(doi: 10.1111/tra.12820)

Single molecule light field microscopy

RR Sims, S Abdul Rehman, MO Lenz, SI Benaissa, E Bruggeman, A Clark, EW Sanders, A Ponjavic, L Muresan, SF Lee, K O’Holleran

Optica

(2020)

1065

(doi: 10.1364/optica.397172)

Distinct domain requirements for EAP45 in HIV budding, late endosomal recruitment, and cytokinesis

B Meng, PPV Ramirez, KM Scherer, E Bruggeman, JC Kenyon, CF Kaminski, AM Lever

bioRxiv

(2020)

2020.05.23.112607

(doi: 10.1101/2020.05.23.112607)

Dr Ezra Bruggeman

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