TheLeeLab

Dr Lisa-Maria Needham

Project: Developing new fluorophores for super-resolution and single-molecule fluorescence Imaging.

Publications

Label-free observation of individual solution phase molecules
L-M Needham, C Saavedra, JK Rasch, D Sole-Barber, BS Schweitzer, AJ Fairhall, CH Vollbrecht, B Mehlenbacher, Z Zhang, L Tenbrake, H Pfeifer, ER Chapman, RH Goldsmith
bioRxiv
(2023)
4
(doi: 10.1101/2023.03.24.534170)
POLCAM: Instant molecular orientation microscopy for the life sciences
E Bruggeman, O Zhang, L-M Needham, M Körbel, S Daly, M Cheetham, R Peters, T Wu, AS Klymchenko, SJ Davis, EK Paluch, D Klenerman, MD Lew, K O’Holleran, SF Lee
(2023)
(doi: 10.1101/2023.02.07.527479)
ThX: a next generation probe for the early detection of amyloid aggregates
L-M Needham, J Weber, JA Varela, JWB Fyfe, D T., CK Xu, D Klenerman, CM Dobson, CA Hunter, SE Bohndiek, TN Snaddon, SF Lee
Biophysical Journal
(2022)
121
(doi: 10.1016/j.bpj.2021.11.2608)
A Comparative Study of High-Contrast Fluorescence Lifetime Probes for Imaging Amyloid in Tissue.
F Gorka, CM Pearson, E Bulovaite, YP Zhang, A Handa, SGN Grant, TN Snaddon, LM Needham, SF Lee
Journal of Physical Chemistry B
(2021)
125
(doi: 10.1021/acs.jpcb.1c07762)
Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer’s R47H mutant increases Aβ aggregation
A Vilalta, Y Zhou, J Sevalle, JK Griffin, K Satoh, DH Allendorf, S De, M Puigdellívol, A Bruzas, MA Burguillos, RB Dodd, F Chen, Y Zhang, P Flagmeier, L-M Needham, M Enomoto, S Qamar, J Henderson, J Walter, PE Fraser, D Klenerman, SF Lee, P St George-Hyslop, GC Brown
Journal of Biological Chemistry
(2021)
296
(doi: 10.1016/j.jbc.2021.100631)
Single-molecule fluorescence detection of a tricyclic nucleoside analogue
GN Samaan, MK Wyllie, JM Cizmic, L-M Needham, D Nobis, K Ngo, S Andersen, SW Magennis, SF Lee, BW Purse
Chemical science
(2020)
12
(doi: 10.1039/d0sc03903a)
Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, while the Alzheimer’s R47H mutant does the opposite
A Vilalta, Y Zhou, J Sevalle, JK Griffin, K Satoh, DH Allendorf, S De, M Puigdellívol, A Bruzas, MA Burguillos, RB Dodd, F Chen, Y Zhang, P Flagmeier, L-M Needham, M Enomoto, S Qamar, J Henderson, J Walter, PE Fraser, D Klenerman, SF Lee, P St George-Hyslop, GC Brown
(2020)
(doi: 10.1101/2020.12.03.409995)
vLUME: 3D virtual reality for single-molecule localization microscopy
S Lee, A Handa, L-M Needham, A Carr, A Ponjavic, J McColl
Nature methods
(2020)
17
(doi: 10.1038/s41592-020-0962-1)
A Comparative Photophysical Study of Structural Modifications of Thioflavin T-Inspired Fluorophores.
L-M Needham, J Weber, CM Pearson, DT Do, F Gorka, G Lyu, SE Bohndiek, TN Snaddon, SF Lee
The Journal of Physical Chemistry Letters
(2020)
11
(doi: 10.1021/acs.jpclett.0c01549)
Single-molecule visualisation of DNA G-quadruplex formation in live cells.
M Di Antonio, A Ponjavic, A Radzevičius, RT Ranasinghe, M Catalano, X Zhang, J Shen, L-M Needham, SF Lee, D Klenerman, S Balasubramanian
Nature Chemistry
(2020)
12
(doi: 10.1038/s41557-020-0506-4)

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Telephone number

01223 336410 (shared)

Email address

lmed2@cam.ac.uk

College

Girton College
Yusuf Hamied Department of Chemistry
Lensfield Road
Cambridge
CB2 1EW
T: +44 (0) 1223 336300
enquiries@ch.cam.ac.uk
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